DETECTION OF MALATHION USING A CARBON NANOTUBE MODIFIED GOLD ELECTRODE


Sulak M. T. , KESKİNLER B.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.20, ss.2744-2749, 2011 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 20
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.aca.2011.06.014
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Sayfa Sayıları: ss.2744-2749

Özet

We developed a simple strategy for designing a sensitive electrochemical biosensor for organophosphate insecticides (OIs), based on an acetylcholinesterase (AChE)-carbon nanotube (CNT)-polypyrrole (PPy) biocomposite film modified gold electrode (labeled as AChE-CNT-PPy/Au). Based on the inhibition of OIs to the enzymatic activity of AChE, using malathion as a model compound, the conditions for detection of the insecticides were explored. The proposed method demonstrates an easy electron transfer between the immobilized enzyme and the electrode via functionalized CNTs in a polypyrrole matrix. Under optimal conditions, the inhibition of malathion was proportional to its concentration from 1.0-20.0 mu M. The detection limit was 0.1 mu M, with a good precision (RSD = 4.7 %, n = 8). The developed biosensor exhibited good stability. This method could be developed as a conventional one to select efficient enzyme inhibitors and investigate toxic compounds against enzymes.