OBJECTIVE: We aimed to investigate the possible effect of topiramate (TOP, 0.02 mg/kg/day) on the livers in
a high-fat diet (HFD)-induced obesity rat model. The other objective was to evaluate the relationship between
TOP administration and NPY level using anti-NPY1R antibody.
METHODS: Twenty-four adult female Wistar albino rats were randomly assigned into four equal groups as
follow: control (CONT), obese (OBS), TOP, and OBS+TOP. All liver samples were investigated using the
stereological analysis, as well as immunohistochemical and histopathological examination.
RESULTS: The total number of hepatocytes was signifi cantly decreased in the OBS+TOP group compared
to the CONT group or the OBS group (p < 0.05). We found a signifi cant increase in the mean volume of liver
in the OBS group compared to the CONT group (p < 0.05). Also, the mean volume of liver was signifi cantly
decreased in the OBS+TOP group compared to the OBS group (p < 0.05).
CONCLUSION: Taken together, our fi ndings suggest that decreased liver volume is possibly attributed to TOP
administration via setting the NPY level in the obese rats. Further, the side effects of TOP in combination with
health risk of obesity may have led to an increase in hepatotoxicity and the subsequent hepatocyte loss (Fig. 7,