THE EFFECT OF DIMETHOATE ON CAPOETA CAPOETA (GULDENSTAEDT 1773) AND THE PROTECTIVE EFFECT OF ASCORBIC ACID


Creative Commons License

Tanrıverdi E. A. , Yılmaz M., Koç E., Akgül A., Ersan Y.

Fresenius Environmental Bulletin, cilt.25, ss.5974-5979, 2016 (SCI Expanded İndekslerine Giren Dergi)

  • Cilt numarası: 25 Konu: 12
  • Basım Tarihi: 2016
  • Dergi Adı: Fresenius Environmental Bulletin
  • Sayfa Sayıları: ss.5974-5979

Özet

In this study, Dimethoate (DM) 􀀋􀁄􀀃􀁓􀁈􀁖􀁗􀁌􀁆􀁌􀁇􀁈􀀌􀂶􀁖􀀃

histopathological and electrophoretic effects were

investigated on Capoeta capoeta (Guldenstaedt

1773) that were captured from Kars creek, and the

protective effect of ascorbic acid (AA) (vitamin C)

was tested. After allowing time for their adaptations

to their new environment, the fish caught from Kars

creek were divided into 4 groups (each including 10

fish), and placed in 300-liter tanks in the laboratory.

The fish were kept in water containing different

solutions: group I in normal water medium, group II

in 100 mg/L AA, group III in 2 mg/L DM, and group

IV in 100 mg/L AA and 2 mg/L DM. They were kept

in these water mediums for 10 days. After this

period, blood and tissue samples were taken from the

fish for electrophoretic and histopathological

studies. Tissue samples were detected in 10%

formaldehyde solution and paraffin blocks were

prepared by routine histological methods. The

samples were cut into sections 3-5 μ thick, all stained

according to hematoxylin and eosin staining

methods, and they were examined under a

microscope. Obtained serum samples were carried

out in Sodium Dodecyl Sulfate Polyacrylamide Gel

Electrophoresis (SDS-PAGE). When we compared

the serum samples with the control group of fish,

protein bands in the application of 100 mg/L AA and

2 mg/L DM, thickening in 36 kD band, thinning in

100 kD, 97 kD, 76 kD, 56 kD and 30 kD bands were

observed, and the bands of 93 kD, 46 kD, 44 kD and

26 kD were detected as newly-synthesized in SDSPAGE

electropherogram. In the histopathological

examinations, depending on DM in intestine, liver,

and kidney tissues obtained from the group formed

by application of DM and AA, degeneration,

necrosis, pyknotic nuclei and cell infiltrates were

detected. As a result, whereas DM application was

found to create toxic effects on Capoeta capoeta, AA

was found to have a healing effect.