Development of Amperometric Biosensor for Phenolic Compounds Using a Modified Electrode with Poly(GMA-co-MTM) and Laccase

Sulak M. T. , ERHAN E., KESKİNLER B., YILMAZ F., Celik A.

SENSOR LETTERS, cilt.8, ss.262-267, 2010 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 8 Konu: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1166/sl.2010.1260
  • Sayfa Sayıları: ss.262-267


A new polymeric matrix, the copolymer of 3-thienylmethyl methacrylate (MTM) and glycidyl methacrylate (GMA), was used to develop a phenol biosensor based on laccase (TvLac). Chemical immobilization of the enzyme, TvLac, was achieved via the epoxy moiety of GMA onto the GMA-co-MTM coated glassy carbon electrode. GC/(GMA-co-MTM)/TvLac working electrode was investigated for the parameters of linear range, sensitivity, stability, pH and detection limit. Amperometric response was measured as a function of concentration of phenolic compounds, at a fixed bias voltage of -200 mV. The sensitivity in the linear range increased in this order with the GC/(GMA-co-MTM)/TvLac: 3-Chlorophenol > 2-Chlorophenol > 2,6-Dimethoxyphenol > Catechol > 4Methoxyphenol > 4-Chlorophenol > Phenol > Hydroquinone > 2,4-Dichlorophenol > p-Benzoquinone. The biosensor exhibited good sensitivity, stability for the tested phenolics compared to the reported working electrodes. It retained 65% of its initial activity after using for 300 measurements in one month.