In this study, the effect of milling time on wear behaviour of the Ti6Al4V alloy produced with the high-energy milling method was investigated. The Ti6Al4V alloy was milled at five different milling times in a mechanical alloying device. The milled powders were cold-pressed under 620 MPa pressure, sintered at 1300 degrees C for 2 h and cooled to room temperature in the furnace. The sintered alloys were characterised with SEM, XRD and hardness and density measurements. Wear tests were performed using a pin-on-disc type wear testing device, under three different loads, at four different sliding distances in simulated body fluid environment. Results showed a decreasing powder size with increasing milling time. The highest decline in size occurred for the powders milled for 120 min. The result of hardness measurements and wear tests showed that samples milled for 120 min had both the highest hardness value and the lowest weight loss.