The aim of this study is to investigate the effects of age and gender in sound reflection of anxiety with acoustic analysis. In the study, 148 speech records that express the emotions of the actors as anxiety and neutral were used as the data set. PRAAT software is used for acoustic analysis. The ANOVA method was used to analyze the data. The according to the results of statistical analysis, gender and age increased the count of acoustic parameters that affected of anxiety. The standard deviation of F0 increased too much, jitter local and jitter rap increased mid-range and other parameters did not change when examined changes based gender. The mean of F0, shimmer apq3 and number of unvoiced frame decreased to mid-range, the standard deviation of F0 and jitter local increased too much, the standard deviation of F3 and jitter rap increased to mid-range and other parameters did not change when examined changes based age. The changes occurring in emotions cause changes in sound by affecting respiratory and muscle tension. The anxiety has been changed according to gender and age because the number of parameters in the analysis based on the gender and age is more. The gender causes change in the speed of glottic cycle and this change increases with anxiety. In addition, vocal cords by both male and female occur irregularities and this case also differs according to age. The irregularities in intensity of sound in lower ages are being further while the pauses in the conversation with advancing age are increasing. (C) 2016 The Authors. Published by IASE. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).